Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. delbrueckii BD7: Identification of potential biological activity

Abstract. Rubak YT, Nuraida L, Iswantini D, Prangdimurti E, Sanam MUE. 2021. Peptide profiling of goat milk fermented by Lactobacillus delbrueckii ssp. BD7: Identification potential biological activity. Biodiversitas 22: 3136-3145. This study investigated the angiotensin-converting enzyme (ACE) inhibitory activity in BD7, characterizing peptide and its as a bioactive peptide. The starter culture (2%) was inoculated into pasteurized skim (11%), then incubated at 37 °C until it reached pH 4.6. Centrifugation 6000 g x 10 minutes 4 applied. supernatant obtained ultrafiltrated using membrane cut-off with molecular weight 3 kDa, fraction analyzed to determine ACE. Peptides were characterized Nano LC / MS MS, identification peptides carried out based on literature review. ACE Lb. BD7 55.98 ± 3.53%. A total 157 released weights ranging from 770.78 - 2081.12 Da having 7-19 amino acid residues. main hydrolyzed casein (72.6%), cleavage parent protein, specific for aliphatic aromatic acids. similarity residues C-terminal 28 inhibitor peptides, 19 antioxidant ten antimicrobial peptides. Some these have homologous sequences previously reported has produce milk, which is rich